Biocontamination dans un Laboratoire de Niveau 1 de Sécurité Biologique à Antananarivo en 2019

ZAKASOA MBOLOLONA RAVAOARISAINA, Vonintsoa Lalaina RAHAJAMANANA, Zely Rivelaine RANDRIAMANANTANY, Andry RASAMINDRAKOTROKA

Abstract


Background: A level 1 biosecurity laboratory is not equipped with a safety device and the benches have no protection against the spread of biological agents. The objective of this study is to identify the most contaminated areas in the working environment in the bacteriology laboratory.

Methods: This study was carried out in the medical analysis laboratory of the Ambohimiandra University Hospital Center in Antananarivo (Madagascar) in 2019. Samples of the environment (air and surfaces) and skin were collected with sterile dry swabs humidified with physiological water. Isolated organisms were studied if they have similar morphology similar to culture media contaminants in examinations of patient samples. Identification and antibiotic susceptibility testing have been done on each isolated bacteria.

Results: During the study, 14 samples were collected, 10 of which were positive, particularly with Staphylococci. Coagulase negative staphylococci were predominant and found on all types of samples (air, surfaces, hands), but more particularly on hands of technicians. Klebsiella pneumoniae and non-fermentative bacteria were also observed respectively in the air samples and surfaces.
Conclusion: The biological contamination of the environment and of the hands of the personnel impacts on the results of biological analyzes hindering the interpretation even leading to the error of the results. Measures corresponding to the biological safety level of the laboratory must be adapted to prevent these errors.
Key words - Biocontamination; bacteria; Hospital hygiene; Laboratory; Biological safety.


Keywords


Biocontamination; bacteria; Hospital hygiene; Laboratory; Biological safety.

Full Text:

PDF

References


P. GARRY. La contamination biologique. Bull. Liaison CTSCCV. 1998 ; 8 (N°3). 157 – 161.

OMS. Manuel de sécurité biologique en laboratoire Troisième édition. Génève. 2005.

OMS. Système de Gestion de la Qualité au Laboratoire. Introduction à la qualité. [Consulté le 10 Aout 2019]. https://extranet.who.int/lqsi/sites/default/files/attachedfiles/LQMS%201%20Intro%20to%20quality_1.pdf

Société Française de Microbiologie. Staphylococcus spp. In : CASFM / EUCAST : Société Française de Microbiologie Ed ; 2018: 57-63.

LEON LE MINOR, MICHEL VERON, Bactériologie Médicale 2ème Edition Paris, 1989, 396- 795p.

Surveillance microbiologique de l’environnement dans les établissements de santé ; Air, eaux et surfaces. Ministère chargé de la santé, DGS/DHOS, CTIN, 2002.

Weber DJ, Rutala WA. Environmental issues and nosocomial infections. In :Wenzel RP. Prevention and control of nosocomial infections (3rd ed). Williams &Wilkins, Baltimore, 1997, 491-514.

CUSSTR Commission Universitaire de Sécurité et Santé au Travail Romande. Dangers et risques biologiques. [Consulté ce 18 Aout 2019]. http://cusstr.ch/repository/133.pdf.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 ZAKASOA MBOLOLONA RAVAOARISAINA

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.