Antimicrobial Potential of Various Essential Oils against Multidrug-Resistant Uropathogenic Escherichia Coli

Menarva Mohamed Moustafa, Zenat Kamel Mohamed, Doaa Mohamed Gheeth, Mohamed Gamal Salah

Abstract


Uropathogenic Escherichia coli is one of the major pathogens causing urinary tract infections. This study aimed to identify the prevalence of antibiotic resistance among pathogenic E. coli isolated from patients with urinary tract infections and evaluation of plant-derived essential oils as alternatives to antibiotics. The majority of isolated strains exhibited remarkable resistance against various antibiotics especially quinolones. Of 100 strains, 78 were found to acquire no susceptibility to at least one antibiotic in three or more classes and classified as multidrug resistant. The antibacterial potential of ten essential oils were investigated and results revealed that clove and oregano were the most powerful essential oils with MIC 0.25 and 1 µL/mL, respectively. The present results suggest that plant-derived EOs may assist in antimicrobial fight, especially those contain high amounts of eugenol and terpenoids (such as clove and oregano) which are active against multidrug-resistant strains.


Keywords


UPEC, Resistant, Quinolone, Antimicrobial

Full Text:

PDF

References


A.L. Flores-Mireles, J.N. Walker, M. Caparon, S.J. Hultgren, Urinary tract infections: epidemiology, mechanisms of infection and treatment options, Nat. Rev. Microbiol. 13 (2015) 269–284. doi:10.1038/nrmicro3432.

B. Owrangi, N. Masters, A. Kuballa, C. O’Dea, T.L. Vollmerhausen, M. Katouli, Invasion and translocation of uropathogenic Escherichia coli isolated from urosepsis and patients with community-acquired urinary tract infection, Eur. J. Clin. Microbiol. Infect. Dis. 37 (2018) 833–839. doi:10.1007/s10096-017-3176-4.

J. Sarowska, B. Futoma-Koloch, A. Jama-Kmiecik, M. Frej-Madrzak, M. Ksiazczyk, G. Bugla-Ploskonska, I. Choroszy-Krol, Virulence factors, prevalence and potential transmission of extraintestinal pathogenic Escherichia coli isolated from different sources: recent reports., Gut Pathog. 11 (2019) 10. doi:10.1186/s13099-019-0290-0.

I. Ali, Z. Rafaque, I. Ahmed, F. Tariq, S.E. Graham, E. Salzman, B. Foxman, J.I. Dasti, Phylogeny, sequence-typing and virulence profile of uropathogenic Escherichia coli (UPEC) strains from Pakistan., BMC Infect. Dis. 19 (2019) 620. doi:10.1186/s12879-019-4258-y.

A. Farajzadah Sheikh, H. Goodarzi, M.J. Yadyad, S. Aslani, M. Amin, N. Jomehzadeh, R. Ranjbar, M. Moradzadeh, S. Azarpira, M.R. Akhond, M. Hashemzadeh, Virulence-associated genes and drug susceptibility patterns of uropathogenic Escherichia coli isolated from patients with urinary tract infection., Infect. Drug Resist. 12 (2019) 2039–2047. doi:10.2147/IDR.S199764.

M. Raeispour, R. Ranjbar, Antibiotic resistance, virulence factors and genotyping of Uropathogenic Escherichia coli strains., Antimicrob. Resist. Infect. Control. 7 (2018) 118. doi:10.1186/s13756-018-0411-4.

M.R. Asadi Karam, M. Habibi, S. Bouzari, Urinary tract infection: Pathogenicity, antibiotic resistance and development of effective vaccines against Uropathogenic Escherichia coli, Mol. Immunol. 108 (2019) 56–67. doi:10.1016/j.molimm.2019.02.007.

M.J. Chavan, D.B. Shinde, S.A. Nirmal, Major volatile constituents of Annona squamosa L. bark, Nat. Prod. Res. 20 (2006) 754–757. doi:10.1080/14786410500138823.

E. Palazzolo, V. Armando Laudicina, M. Antonietta Germanà, Current and Potential Use of Citrus Essential Oils, Curr. Org. Chem. 17 (2013) 3042–3049. doi:10.2174/13852728113179990122.

R.S. Borges, B.L.S. Ortiz, A.C.M. Pereira, H. Keita, J.C.T. Carvalho, Rosmarinus officinalis essential oil: A review of its phytochemistry, anti-inflammatory activity, and mechanisms of action involved, J. Ethnopharmacol. 229 (2019) 29–45. doi:10.1016/j.jep.2018.09.038.

H.S. Elshafie, I. Camele, An Overview of the Biological Effects of Some Mediterranean Essential Oils on Human Health, Biomed Res. Int. 2017 (2017) 9268468. doi:10.1155/2017/9268468.

Y.R. Nairoukh, A.M. Mahafzah, A. Irshaid, A.A. Shehabi, Molecular Characterization of Multidrug Resistant Uropathogenic E. Coli Isolates from Jordanian Patients., Open Microbiol. J. 12 (2018) 1–7. doi:10.2174/1874285801812010001.

The Clinical and Laboratory Standards Institute, Performance Standards for Antimicrobial Susceptibility Testing CLSI supplement M100S, 2016.

S.A. Ochoa, A. Cruz-Córdova, V.M. Luna-Pineda, J.P. Reyes-Grajeda, V. Cázares-Domínguez, G. Escalona, M.E. Sepúlveda-González, F. López-Montiel, J. Arellano-Galindo, B. López-Martínez, I. Parra-Ortega, S. Giono-Cerezo, R. Hernández-Castro, D. de la Rosa-Zamboni, J. Xicohtencatl-Cortes, Multidrug- and Extensively Drug-Resistant Uropathogenic Escherichia coli Clinical Strains: Phylogenetic Groups Widely Associated with Integrons Maintain High Genetic Diversity, Front. Microbiol. 7 (2016) 2042. doi:10.3389/fmicb.2016.02042.

A. Man, L. Santacroce, R. Jacob, A. Mare, L. Man, Antimicrobial Activity of Six Essential Oils Against a Group of Human Pathogens: A Comparative Study, Pathogens. 8 (2019) 15. doi:10.3390/pathogens8010015.

S.A. Ochoa, A. Cruz-Córdova, V.M. Luna-Pineda, J.P. Reyes-Grajeda, V. Cázares-Domínguez, G. Escalona, M.E. Sepúlveda-González, F. López-Montiel, J. Arellano-Galindo, B. López-Martínez, I. Parra-Ortega, S. Giono-Cerezo, R. Hernández-Castro, D. de la Rosa-Zamboni, J. Xicohtencatl-Cortes, Multidrug- and Extensively Drug-Resistant Uropathogenic Escherichia coli Clinical Strains: Phylogenetic Groups Widely Associated with Integrons Maintain High Genetic Diversity, Front. Microbiol. 7 (2016) 2042. doi:10.3389/fmicb.2016.02042.

O. Fasugba, A. Gardner, B.G. Mitchell, G. Mnatzaganian, Ciprofloxacin resistance in community- and hospital-acquired Escherichia coli urinary tract infections: a systematic review and meta-analysis of observational studies, BMC Infect. Dis. 15 (2015) 545. doi:10.1186/s12879-015-1282-4.

D. Maron, T.J. Smith, K.E. Nachman, Restrictions on antimicrobial use in food animal production: an international regulatory and economic survey, Global. Health. 9 (2013) 48. doi:10.1186/1744-8603-9-48.

H. Mith, R. Duré, V. Delcenserie, A. Zhiri, G. Daube, A. Clinquart, Antimicrobial activities of commercial essential oils and their components against food-borne pathogens and food spoilage bacteria., Food Sci. Nutr. 2 (2014) 403–16. doi:10.1002/fsn3.116.

A.A. Tayel, H. Hussein, N.M. Sorour, W.F. El-Tras, Foodborne Pathogens Prevention and Sensory Attributes Enhancement in Processed Cheese via Flavoring with Plant Extracts., J. Food Sci. 80 (2015) M2886-91. doi:10.1111/1750-3841.13138.

C. Valcourt, P. Saulnier, A. Umerska, M.P. Zanelli, A. Montagu, E. Rossines, M.L. Joly-Guillou, Synergistic interactions between doxycycline and terpenic components of essential oils encapsulated within lipid nanocapsules against gram negative bacteria., Int. J. Pharm. 498 (2016) 23–31. doi:10.1016/j.ijpharm.2015.11.042.

R. Requena, M. Vargas, A. Chiralt, Eugenol and carvacrol migration from PHBV films and antibacterial action in different food matrices., Food Chem. 277 (2019) 38–45. doi:10.1016/j.foodchem.2018.10.093.

D.H. Gilling, S. Ravishankar, K.R. Bright, Antimicrobial efficacy of plant essential oils and extracts against Escherichia coli., J. Environ. Sci. Health. A. Tox. Hazard. Subst. Environ. Eng. 54 (2019) 608–616. doi:10.1080/10934529.2019.1574153.

J.K. Kovács, P. Felső, L. Makszin, Z. Pápai, G. Horváth, H. Ábrahám, T. Palkovics, A. Böszörményi, L. Emődy, G. Schneider, Antimicrobial and Virulence-Modulating Effects of Clove Essential Oil on the Foodborne Pathogen Campylobacter jejuni., Appl. Environ. Microbiol. 82 (2016) 6158–6166. doi:10.1128/AEM.01221-16.

J. Vergis, P. Gokulakrishnan, R.K. Agarwal, A. Kumar, Essential Oils as Natural Food Antimicrobial Agents: A Review, Crit. Rev. Food Sci. Nutr. 55 (2015) 1320–1323. doi:10.1080/10408398.2012.692127.

M. De Vincenzi, A. Stammati, A. De Vincenzi, M. Silano, Constituents of aromatic plants: carvacrol, Fitoterapia. 75 (2004) 801–804. doi:10.1016/j.fitote.2004.05.002.

I. Rodriguez-Garcia, B.A. Silva-Espinoza, L.A. Ortega-Ramirez, J.M. Leyva, M.W. Siddiqui, M.R. Cruz-Valenzuela, G.A. Gonzalez-Aguilar, J.F. Ayala-Zavala, Oregano Essential Oil as an Antimicrobial and Antioxidant Additive in Food Products, Crit. Rev. Food Sci. Nutr. 56 (2016) 1717–1727. doi:10.1080/10408398.2013.800832.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 Menarva Mohamed Moustafa, Zenat Kamel Mohamed, Doaa Mohamed Gheeth, Mohamed Gamal Salah

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.