Intestinal Giardiasis at UPFR Parasitology-Mycology of CHU-JRA Antananarivo, Madagascar

Tsatoromila Fenosoa Anita Mireille, Norosoa Julie ZAFINDRAIBE, Elodie Emile BATAVISOANIATSY, Isabelle Francine Rakotonindrina, Christian Rafalimanana, Aimée Olivat RAKOTO ALSON, Lala Rasoamialy-Soa RAZANAKOLONA

Abstract


Introduction: Giardiasis is an intestinal parasitosis extremely widespread in the world but under-diagnosed in Madagascar. It is often asymptomatic. It can be fatal due to intestinal malabsorption syndrome. This study was conducted to determine the epidemiological-clinical aspect of giardiasis in Antananarivo and to determine the relevance of prescribing the KAOP stool examination for its diagnosis.

Materials and methods: We report the results of a retrospective study of 2154 KAOP stool examinations performed at the Parasitology-Mycology Laboratory of the CHU-JRA over a 13-year period from June 2005 to June 2018.

Results. The positivity rate for intestinal parasitosis was 58.36% or 1257 out of 2154 files examined. We found a prevalence of 1.76% (38/2154) of all claims and a frequency of 3.2% (38/1257) compared to other parasitoses. We found a female predominance with a sex ratio of 0.8.  The average age was 20.46 years, of which 39.47%  (15/38) were under 10 years of age.  Abdominal pain were the main reasons for requesting tests. Only the cystic form of Giardia duodenalis was found, of which 28.95% (11 cases) were isolated and 71.05% (27) were associated with one or more parasites.

Conclusion:  Giardiasis is a parasitosis with a harmful effect on children. It is still under-diagnosed in Madagascar. Compliance with the prescription of 3 successive stool examinations allows a diagnosis of certainty.


Keywords


KAOP Stool Examination - Frequency - Giardia – Epidemiology.

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References


Laishram S, Kang G, Ajjampur SSR. Giardiasis: a review on assemblage distribution and epidemiology in India. Indian J Gastroenterol Off J Indian Soc Gastroenterol. janv 2012;31(1):3 1

Savioli L, Smith H, Thompson A. Giardia and Cryptosporidium join the ‘Neglected Diseases Initiative’. Trends Parasitol. 1 mai 2006;22(5):203 8.

Soares R, Tasca T. Giardiasis: an update review on sensitivity and specificity of methods for laboratorial diagnosis. J Microbiol Methods. 1 oct 2016;129:98 102

Fischer Walker CL, Aryee MJ, Boschi-Pinto C, Black RE. Estimating Diarrhea Mortality among Young Children in Low and Middle Income Countries. Myer L, éditeur. PLoS ONE. 3 janv 2012;7(1):e29151.

Feng Y, Xiao L. Zoonotic Potential and Molecular Epidemiology of Giardia Species and Giardiasis. Clin Microbiol Rev. janv 2011;24(1):110 40

Al-Mekhlafi HM, Al-Maktari MT, Jani R, Ahmed A, Anuar TS, Moktar N, et al. Burden of Giardia duodenalis Infection and Its Adverse Effects on Growth of Schoolchildren in Rural Malaysia. PLoS Negl Trop Dis [Internet]. 31 oct 2013 [cité 4 sept 2019];7(10). Disponible sur: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814875/

Pubmeddev, al GV et. Human intestinal parasites in Mahajanga, Madagascar: The kingdom of the protozoa. - PubMed - NCBI [Internet]. [cité 6 sept 2019]. Disponible sur: https://www.ncbi.nlm.nih.gov/pubmed/30304028

Randremanana R, Randrianirina F, Gousseff M, Dubois N, Razafindratsimandresy R, Hariniana ER, et al. Case-Control Study of the Etiology of Infant Diarrheal Disease in 14 Districts in Madagascar. PLOS ONE. 17 sept 2012; 7(9):e44533

Oliveira D, Ferreira FS, Atouguia J, Fortes F, Guerra A, Centeno-Lima S. Infection by Intestinal Parasites, Stunting and Anemia in School-Aged Children from Southern Angola. Weber CR, éditeur. PLOS ONE. 15 sept 2015;10(9):e0137327.

Breurec S, Vanel N, Bata P, Chartier L, Farra A, Favennec L, et al. Etiology and Epidemiology of Diarrhea in Hospitalized Children from Low Income Country: A Matched Case-Control Study in Central African Republic. PLoS Negl Trop Dis [Internet]. 5 janv 2016;10(1). Disponible sur: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4701495/

Berrilli F, Di Cave D, N’Guessan R, Kaboré Y, Giangaspero A, Sorge RP, et al. Social determinants associated with Giardia duodenalis infection in southern Côte d’Ivoire. Eur J Clin Microbiol Infect Dis. 1 oct 2014;33(10):1799 802.

El-Shabrawi M, Salem M, Abou-Zekri M, El-Naghi S, Hassanin F, El-Adly T, et al. The burden of different pathogens in acute diarrhoeal episodes among a cohort of Egyptian children less than five years old. Przegla̜d Gastroenterol. 2015;10(3):173 80.

Wegayehu T, Karim MR, Li J, Adamu H, Erko B, Zhang L, et al. Multilocus genotyping of Giardia duodenalis isolates from children in Oromia Special Zone, central Ethiopia. BMC Microbiol [Internet]. 21 mai 2016 [cité 4 sept 2019];16. Disponible sur: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4875739/

Ferreira FS, Centeno-Lima S, Gomes J, Rosa F, Rosado V, Parreira R, et al. Molecular characterization of Giardia duodenalis in children from the Cufada Lagoon Natural Park, Guinea-Bissau. Parasitol Res. 1 nov 2012;111(5):2173 7.

Mbae CK, Nokes DJ, Mulinge E, Nyambura J, Waruru A, Kariuki S. Intestinal parasitic infections in children presenting with diarrhoea in outpatient and inpatient settings in an informal settlement of Nairobi, Kenya. BMC Infect Dis. 27 mai 2013; 13: 243.

Rahouma A, Klena JD, Krema Z, Abobker AA, Treesh K, Franka E, et al. Enteric Pathogens Associated with Childhood Diarrhea in Tripoli-Libya. Am J Trop Med Hyg. 1 juin 2011; 84(6):886 91.

Ross AGP, Olds GR, Cripps AW, Farrar JJ, McManus DP. Enteropathogens and Chronic Illness in Returning Travelers. N Engl J Med. 9 mai 2013;368(19):1817 25.

Soares R, Tasca T. Giardiasis: an update review on sensitivity and specificity of methods for laboratorial diagnosis. J Microbiol Methods. 1 oct 2016;129:98 102.

Bouratbine A, Aoun K, Siala E, Chahed MK, Ben Hassine L , Meherzi A. Pour une meilleure estimation de la prévalence du parasitisme intestinal dans la région de Tunis. Bull Soc Pathol Exot, 2000, 93, 5, 353-35

Hamdan I. Al-Mohammed. Genotypes of Giardia intestinalis clinical isolates of gastrointestinal symptomatic and asymptomatic; Parasitol Res (2011) 108:1375–1381Saudi children

Breathnach AS, McHugh TD, Butcher PD. Prevalence and clinical correlations of genetic subtypes of Giardia lamblia in an urban setting. Epidemiol Infect. oct 2010;138(10):1459 67

Ajjampur SSR, Koshy B, Venkataramani M, Sarkar R, Joseph AA, Jacob KS, et al. Effect of cryptosporidial and giardial diarrhoea on social maturity, intelligence and physical growth in children in a semi-urban slum in south India. Ann Trop Paediatr. 2011; 31(3):205 12.

Nematian J, Gholamrezanezhad A, Nematian E. Giardiasis and other intestinal parasitic infections in relation to anthropometric indicators of malnutrition: a large, population-based survey of schoolchildren in Tehran. Ann Trop Med Parasitol. avr 2008;102(3):209 14.

McHardy IH, Wu M, Shimizu-Cohen R, Couturier MR, Humphries RM. Detection of Intestinal Protozoa in the Clinical Laboratory. J Clin Microbiol. 1 mars 2014; 52(3):712 20.

Gotfred-Rasmussen H, Lund M, Enemark HL, Erlandsen M, Petersen E. Comparison of sensitivity and specificity of 4 methods for detection of Giardia duodenalis in feces: immunofluorescence and PCR are superior to microscopy of concentrated iodine-stained samples. Diagn Microbiol Infect Dis. 2016 Mar;84(3):187-90. doi: 10.1016/j.diagmicrobio.2015.11.005. Epub 2015 Nov 10


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