The Expression Analysis of KLF11, PCDH9 and TGF-Β2 Genes in Patients with Non-Small Cell Lung Cancer

Mohammad Abdolrezaei Anary, Sajad Nooshin, Arash Matin Ahmadi, Alireza Tavakolpour Negari, Alireza Emamvirdizadeh, Mojtaba Mohammadnejad pahmadani, Shohre Zare Karizi

Abstract


Abstract

Background: Lung cancer remains the leading cause of cancer mortality in men and women in the worldwide. Studies have confirmed the presence of multiple and effective genes such as KLF11, PCDH9 and TGF-β2 can be involved in lung cancer. KLFs are transcriptional regulators that contribute to a wide range of cellular processes. PCDH9 gene belongs to the protocadherins family and its role is cell adhesion and TGF-β activates several proteins and signaling cascades including SMAD proteins and MAPK cascade. So, the aim of this study is to examine the expression of these genes.

Method: In this study, the expression of KLF11, PCDH9 and TGF-β2 genes were checked by Quantitative real time PCR in 30 NSCLC tissue samples and adjacent normal tissue samples.

Results: The results of these genes indicated that the expression level in tumor samples is not significantly different with normal samples (P>0.05). However, this study was designed as a pilot study, and further investigations are required to confirm our findings.


Keywords


Lung cancer; KLF11; PCDH9; TGF-β2; Real Time-PCR; NSCLC.

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References


References

Tanaka, K., K. Kumano, and H. Ueno, Intracellular signals of lung cancer cells as possible therapeutic targets. Cancer science, 2015. 106(5): p. 489-496.

Devarakonda, S., D. Morgensztern, and R. Govindan, Genomic alterations in lung adenocarcinoma. The lancet oncology, 2015. 16(7): p. e342-e351.

Shackelford, R.E., et al., ALK-rearrangements and testing methods in non-small cell lung cancer: a review. Genes & cancer, 2014. 5(1-2): p. 1.

Siegel, R.L., K.D. Miller, and A. Jemal, Cancer statistics, 2016. CA: a cancer journal for clinicians, 2016. 66(1): p. 7-30.

Yu, X.Q., et al., Contrasting temporal trends in lung cancer incidence by socioeconomic status among women in New South Wales, Australia, 1985–2009. Lung Cancer, 2017. 108: p. 55-61.

Cruz, C.S.D., L.T. Tanoue, and R.A. Matthay, Lung cancer: epidemiology, etiology, and prevention. Clinics in chest medicine, 2011. 32(4): p. 605-644.

Alberg, A.J. and J.M. Samet, Epidemiology of lung cancer. Chest, 2003. 123(1): p. 21S-49S.

Chen, G., et al., Synergistic effect of afatinib with su11274 in non-small cell lung cancer cells resistant to gefitinib or erlotinib. PloS one, 2013. 8(3): p. e59708.

Chen, X., et al., Gefitinib or erlotinib as maintenance therapy in patients with advanced stage non-small cell lung cancer: a systematic review. PloS one, 2013. 8(3): p. e59314.

Gazala, S., et al., A systematic review and meta-analysis to assess patient-reported outcomes after lung cancer surgery. The Scientific World Journal, 2013. 2013.

Wang, G., et al., Promoter DNA methylation is associated with KLF11 expression in epithelial ovarian cancer. Genes, Chromosomes and Cancer, 2015. 54(7): p. 453-462.

Wang, C., et al., Characterizing the role of PCDH9 in the regulation of glioma cell apoptosis and invasion. Journal of Molecular Neuroscience, 2014. 52(2): p. 250-260.

Pearson, R., et al., Krüppel-like transcription factors: a functional family. The international journal of biochemistry & cell biology, 2008. 40(10): p. 1996-2001.

Kaczynski, J., T. Cook, and R. Urrutia, Sp1-and Krüppel-like transcription factors. Genome biology, 2003. 4(2): p. 206.

Redies, C., K. Vanhalst, and F. Van Roy, δ-Protocadherins: unique structures and functions. Cellular and Molecular Life Sciences CMLS, 2005. 62(23): p. 2840-2852.

Kahr, I., K. Vandepoele, and F. Van Roy, Delta-protocadherins in health and disease, in Progress in molecular biology and translational science. 2013, Elsevier. p. 169-192.

De Caestecker, M., The transforming growth factor-β superfamily of receptors. Cytokine & growth factor reviews, 2004. 15(1): p. 1-11.

Rahimi, R.A. and E.B. Leof, TGF‐β signaling: A tale of two responses. Journal of cellular biochemistry, 2007. 102(3): p. 593-608.

Hachim, M.Y., et al., Differential expression of TGFβ isoforms in breast cancer highlights different roles during breast cancer progression. Tumor Biology, 2018. 40(1): p. 1010428317748254.

Ma, G.-F., et al., Transforming growth factor-β1 and-β2 in gastric precancer and cancer and roles in tumor-cell interactions with peripheral blood mononuclear cells in vitro. PloS one, 2013. 8(1): p. e54249.

Potapova, A., et al., Epigenetic inactivation of tumour suppressor gene KLF11 in myelodysplastic syndromes. European journal of haematology, 2010. 84(4): p. 298-303.

Ellenrieder, V., TGFβ-regulated gene expression by Smads and Sp1/KLF-like transcription factors in cancer. Anticancer Research, 2008. 28(3A): p. 1531-1539.

Lv, J., et al., PCDH9 acts as a tumor suppressor inducing tumor cell arrest at G0/G1 phase and is frequently methylated in hepatocellular carcinoma. Molecular medicine reports, 2017. 16(4): p. 4475-4482.

Chen, Y., et al., Loss of PCDH9 is associated with the differentiation of tumor cells and metastasis and predicts poor survival in gastric cancer. Clinical & experimental metastasis, 2015. 32(5): p. 417-428.

Nooshin, S., et al., The Methylation Analysis of KLF11 and PCDH9 Genes in Patients with Non-Small Cell Lung Cancer. Cancer and Clinical Oncology, 2018. 7(1): p. 47.


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Copyright (c) 2018 Mohammad Abdolrezaei Anary, Sajad Nooshin, Arash Matin Ahmadi, Alireza Tavakolpour Negari, Alireza Emamvirdizadeh, Mojtaba Mohammadnejad, Shohre Zare Karizi

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