Assessing Methylation of Mir-146b in Patients with Non-Small Cell Lung Cancer

Masoumeh Masrouri, Elham sadat Hosseini manesh, Mahsa Sadeghiazad, Morteza Karimipoor, Shohreh Zare Karizi

Abstract


Background: One of the most important epigenetic factors in lung cancer is aberrant DNA methylation. So far, many studies have been performed on the methylation of various genes and microRNAs in various cancers, especially lung cancer. So because of the high importance of microRNAs in cancer. In this study, mir-146b methylation was checked in NSCLC tissues.

Method: Analysis methylation of mir146b was investigated in 30 samples NSCLC tissues and 30 adjacent normal tissues using by MS-HRM method.

Results: Study on mir146b methylation showed that there was no significant difference between the methylation levels of this microRNA in tumor samples compared with healthy samples (P>0.05). However, this study was designed as a pilot study, and further investigations are required to confirm our findings.


Keywords


Lung Cancer; NSCLC; Mir-146b; MS-HRM Method.

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