Enhancement of Phytochemical Analysis of Tribulus Terrestris Harvested in Tunisia and Marketed as Dietary-Supplement in Developed Countries

Salwa Bouabdallah, Hasim Kelebek, Meriem Azzouz, Mossadok Ben-attia, Hela Ouarda El-ferchichi


The aim of the present study was to detect, quantify and identify the phenolic compounds in Tribulus terrstris (used as remedy in infertility disorder by Ayurveda therapy) extract hydrolyzed by HCL, using high-performance liquid chromatography (HPLC) to detect and quantify non-volatiles compounds. Gas-chromatography coupled to mass spectrometry (GC-MS) was performed to detect and quantify volatile-compounds. A total of fifty (50), eighteen (18), and twenty (20) compounds from Tribulus extract, respectively in seeds, leaves and roots were identified as belonging to various structural classes, mainly phenolic compounds. Analysis by HPLC finger print allowed the identification of procatechic-acid in leaves part detected with high peak area (8.2 mg/g MS).

Keywords: Dietary-supplement; Nutraceutical; Phenolic-compounds; Procathechic-acid; Separation-process; RP-HPLC


Dietary-supplement; Nutraceutical; Phenolic-compounds; Separation-process; Ayurveda

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