Assessing methylation of ATG5 and LC3 related to Autophagy in patients with breast cancer

Mojtaba Mohammadnejad pahmadani, Raheleh Safaejavan, Shohre Zare Karizi

Abstract


Background: Breast Cancer is one of the leading causes of cancer deaths among women worldwide. The role of epigenetics as a distinct mechanism to alter gene expression in a tissue-specific manner has emerged as an important mechanism in the pathophysiology of cancer. One of the most important of epigenetic factor is DNA methylation which has been studied in various cancers.

Method: The methylation specific high resolution melting (MS-HRM) method was used for promoter methylation status investigation of ATG5 and LC3 genes in 30 breast tumor samples and 30 adjacent normal samples.

Results: For the ATG5 and LC3 genes, were methylated 2 (6.67%) of tumor samples and 1 (3.34%) of tumor samples out of max 30 tumor samples, respectively. Also, the rest of the samples showed no methylation change in their promoter region, and statistical results demonstrated that there is no significant difference between the promoter methylation status of these genes in tumor samples and normal samples (P>0.05).


Keywords


Breast Cancer, DNA Methylation, MS-HRM Method, Autophagy, ATG5, LC3

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