Instrumentation System Of Flavonoid Compounds Of Moringa Leaf Using Ultrasound Assisted Extraction (UAE)

Salim Rahmatullah, Suryono Suryono, Jatmiko Endro Suseno


In this study, a computer interface-based temperature monitoring system was built in the Moringa Olivera Lamk leaf extraction process using Ultrasound Assisted Extraction (UAE) to obtain flavonoid compounds. The extraction temperature is monitored during the extraction process. Moringa leaf extraction with UAE was carried out to see the effect of solvent volume and extraction time. The variations carried out are the volume of the solvent in the range 10-30 mL, the extraction time for 10-50 minutes. Methanol was chosen as the solvent in this study using 10 grams of Moringa leaves. Test the total content of flavonoids using UV-VIS spectrophotometry. The temperature monitoring system during the extraction process can be viewed through a computer interface programming. While the results of the extraction of flavonoids from Moringa leaves using UAE showed the best results were obtained using a solvent volume of 20 mL and extraction time of 20 minutes. The best flavonoid content obtained was 2.855 / mg Quarsetin. These results indicate that the solvent volume and extraction time affect the extraction yield of flavonoids from Moringa leaves using Ultrasound Assisted Extraction (UAE).


Temperature Monitoring, Computer Interface, Ultrasound Assisted Extraction (UAE), Moringa Leaves, and Flavonoids.

Full Text:



Safitri, The effect of giving Moringa leaf stew on blood sugar levels in patients with DM type 2 in Bangkinang Urban Village, the working area of the Community Health Center, Ners, 2017, 2(2), pp. 43–50.

Ojha, K.S., Aznar, R., O'donel, C., Tiwari, B.K., Ultrasound technology for the extraction of biologically active molecules from plant, animal and marine sources’, TrAC - Trends in Analytical Chemistry, (2020), 122. doi: 10.1016/j.trac.2019.115663.

Jovanović, A.A., Dordevic, V.B., Zdunic, G.M., Pljevljakusic, D.S., Savikin, K.P., Godevac, D.M., Bugarski, B.M., Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques’, Separation and Purification Technology, (2017), 179, pp. 369–380. doi:


Ayu, H.R., Suryono, S., Suseno, J.E., ‘Determination of the ultrasound power effects on flavonoid compounds from Psidium guajava L. using ANFIS’, Journal of Physics: Conference Series, (2018), 1025(1). doi: 10.1088/1742-6596/1025/1/012024.

Wang, Y., Gao, Y., Ding, H., Liu, S., Han, X., Gui, J., Liu, D., ‘Subcritical ethanol extraction of flavonoids from Moringa oleifera leaf and evaluation of antioxidant activity’, Food Chemistry. Elsevier Ltd, (2017), 218, pp. 152–158. doi: 10.1016/j.foodchem.2016.09.058.

Li, H., Chen, B., and Yao, S., ‘Application of ultrasonic technique for extracting chlorogenic acid from Eucommia ulmodies Oliv. (E. ulmodies)’, Ultrasonics Sonochemistry, (2005), 12(4), pp. 295–300. doi: 10.1016/j.ultsonch.2004.01.033.

Yonaka, B., Dani, D., Wahidah, B.F., Syaifudin, A., ‘Ethnobotany of Moringa oleifera Lam. Plants in Kedungbulus Village, Gembong Pati’, (2019), 2(2), pp. 44–52. doi: 10.21580/ah.v2i2.4659.

Lin M.,, Zhang J., Chen X., Bioactive flavonoids in Moringa oleifera and their health-promoting properties Journal of Functional Foods, 2018, 47 (2018) 469–479.

Keil, F.J., Modeling of Process Intensification. In Alupului, A., Ioan Calinescu, and Vasile Lavric. 2009. Ultrasonic Vs. Microwave Extraction Intensification of Active Principles From Medicinal Plants. AIDIC Conference Series, 2007, Vol. 9 2009 page 1-8.

Liu, Q.M., Yang, X.M., Zhang, L., Majetich, G., Optimization of Ultrasonic-assisted extraction of chlorogenic acid from Folium eucommiae and evaluation of its antioxidant activity. Journal of Medicinal Plants Research, 2010, Vol. 4(23), pp. 2503-2511.

Isnan, W., and Muin, N., ‘Various Benefits of Moringa oleifera Lamk Plants for the Community’, Info Teknis EBONI, (2017), 14(1), pp. 63–75.

Xue Lin X., Lingfeng Wu L., Xiong Wang X., Linling Yao L., Wang L., Ultrasonic-assisted extraction for flavonoid compounds content and antioxidant activities of India Moringa oleifera L. leaves: Simultaneous optimization, HPLC characterization and comparison with other methods, Journal of Applied Research on Medical and Aromatic Plants, 2020,

Suryani N.C., Permana D.G.M., Jambe A.A.G.N.A., Effect of Type of Solvent on Total Flavonoid Content and Antioxidant Activity of Matoa Leaf Extract, Journal of Food Science and Technology, (ITEPA), [S.l.], v. 5, n. 1, june 2016. ISSN 2527-8010.

Chemat F., Rombaut N., Sicaire A.G., Meullemiestre A., Tixier A.S.F., Vian M.A., Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review Ultrasonics Sonochemistry 34 (2017) 540–560.

Suryono, S., Hadiyanto, H., Yasin, M., Widyowati, R., Muniroh, M., Amalia, A., Effect of frequency, temperature, and time of sonication on xanton content of mangosteen (Garcinia mangostana L.) peel extract through ultrasound assisted extraction’ E3S Web of Conferences, (2019), 125(201 9), pp. 9–11. doi: 10.1051/e3sconf/201912525006.

Giancoli, D.C., Physics Fifth Edition Volume 1 (Translate: Hanum, Yuhilza), 2001, Jakarta: Erlangga.



  • There are currently no refbacks.

Copyright (c) 2021 salim rahmatullah, Suryono suryono, Jatmiko Endro Suseno

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.