Optimization Of Antibiotic Production On Various Protein Substrates Against Staphylococcus aureus And Escherichia coli

Hanif Aulia Defnur, Nurmiati Nurmiati, Periadnadi Periadnadi


Optimization has been carried out on the protein substrate, isolates of Slaughterhouse Waste. This study aims to determine the best protein substrate for potential antimicrobial isolates (Bacillus, Isolate Slaughterhouse Waste) in the production of antibiotics against the test bacteria S. aureus and E. coli. This study used an experimental method to optimize each of the different protein substrates in the production of antibiotics from potential isolates from the Slaughterhouse Waste tract against S. aureus and E. coli. From the results of the study, skim milk was obtained as the best substrate because it had the highest Halozone diameter of 15 mm against the test bacteria S. aureus and 9 mm against E. coli. Inhibition against S. aureus was strong, while for E. coli it was moderate.



Antibiotics, Genus Bacillus, Skimmed Milk, Substrate Optimization

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Adriani, Y. and F.Tulak. 2013. Isolation And Characterization Of Actinomycetes as Producers Of Antibiotics From Soil Samples On Cattle Farms In Galesong District, Takalar Regency. Journal of Biogenesis. ISSN : 2302-1616 Vol 1. No. 2 pp. 97-100.

Aminy, S.L. 2019. Efforts to Find Antibiotic Bacteria From Chicken Slaughterhouse Waste Through Screening And In Vitro Testing. Essay. Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University. Padang.

Defnur, H.A. 2019. Isolation, Potential And Characterization Of Antibiotic Bacteria From Slaughterhouse Waste Against Staphylococcus aureus Rosenbach. Essay. Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University. Padang.

Ganiswarna, S.G. 1995. Pharmacology And Therapy, Edition 4. University of Indonesia. Jakarta.

Hansel, R. 1980. Pharmazeutische Biologie. Springer-Verlag Berlin Helderberg New York.

Hatmanti, A. 2000. Introduction of Bacillus spp. Research and Development of the marine environment of LIPI. 15(1):31-41. Jakarta.

Klewing, A., B. M. Koo, L. Kruger, A. Poehlein, D. Reub, R. Daniel, C. A. Gross and J. Stulke. 2020. Resistance To Serine In Bacillus subtilis: Identification Of The Serine Transporter YbeC And Of A Metabolic Network That Links Serine and Threonine Metabolism. Environmental Microbiology (2020) 22(9), 3937–3949.

Magan, J. B., T. F. O. Callaghan, J. Zheng, L. Zhang, R. Mandal, D. Hennessy, M. A. Fenelon, D. S. Wishart, A. L. Kelly and N.A.M.Carthy. 2019. Impact of Bovine Diet On Metabolomic Profile Of Skim Milk And Whey Protein Ingredients. Article Metabolites 2019, 9, 305.

Meevootisom, V., P. Somsuk, R. Prachartam and L. W. Feegel. 1983. Simple Screening Method For Isolation Of Penicillin Acylase Producing Bacteria, Environ. Microb., 46, pp. 1227-1229.

Park, Y.O and M.S. Nam. 2015. Bioactive Peptides in Milk and Dairy Products: A Review. Korean J. Food Science. an. Vol. 35, No. 6, pp. 831~840(2015).

Rohan, H.H., K. Rokhmad, N.L.P.E. Sudiwati and I.R. Rohana. 2016. Basic Microbiology. Issue 1. Deepublish. Yogyakarta.

Sumantri, C., E. Andreas, A. Farajallah and Jarmuji. 2008. κ-Casein Gene Diversity And Its Relationship To Milk Production And Quality In Sheep In The Jonggol Education And Research Unit (UP3). Journal of Agricultural Sciences. ISSN 0853-4217.

DOI: http://dx.doi.org/10.52155/ijpsat.v28.2.3472


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