The expression level of Mir-210 and Mir-141 in breast cancer patients

Shima Hojabri Mahani, Saeedeh Hosseini abnavi, Roya Zarezadeh, Mojtaba Mohammadnejad Pahmadani, Zoofa Zayani, Maryam Nooshin


Background: Breast cancer is a clinically heterogeneous disease. Molecular classification of breast cancer has been proposed based on gene expression profiles of human tumors. Luminal, basal-like, normal-like, and erbB2+ subgroups were identified and were shown to have different prognoses. Breast cancer is the most common cancer among women worldwide, with 1.3 million women diagnosed each year and about 500,000 deaths per year from the disease. Recently molecular studies have been conducted on early diagnosis of breast cancer. In this study, expression levels of Mir-210 and Mir-141 were reported in breast cancer samples.    Methods: The expression levels of Mir-210 and Mir-141 were checked by Real Time-PCR method in 35 breast cancer tissues and 35 adjacent normal tissues.  Results: This study for Mir-210 reported that 42.8% of tumor samples have increased of expression levels comparison with normal samples and 45.8% of tumor samples showed an increase in expression level for Mir-141. Finally, was not observed significant difference between the levels of expression in these microRNAs (P>0.05).


Breast Cancer, Mir-210, Mir-141, Real Time-PCR Method.

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Anderson, W. F., Chu, K. C., Chatterjee, N., Brawley, O., & Brinton, L. A. (2001). Tumor variants by hormone receptor expression in white patients with node-negative breast cancer from the surveillance, epidemiology, and end results database. Journal of Clinical Oncology, 19(1), 18-27.

Bidoki, S. H., Bayatani, A., Sarlak, M., Rasouli, M., Mostafaie, P., Saghafi, M., & Negari, A. T. (2018). Assessing Expression of TGF-Β2 and PCDH9 Genes in Breast Cancer Patients. Age, 7, 20.

Crosby, M. E., Kulshreshtha, R., Ivan, M., & Glazer, P. M. (2009). MicroRNA regulation of DNA repair gene expression in hypoxic stress. Cancer research, 69(3), 1221-1229.

Ding, L., Yu, L. L., Han, N., & Zhang, B. T. (2017). miR-141 promotes colon cancer cell proliferation by inhibiting MAP2K4. Oncology letters, 13(3), 1665-1671.

Esquela-Kerscher, A., & Slack, F. J. (2006). Oncomirs—microRNAs with a role in cancer. Nature Reviews Cancer, 6(4), 259.

Finlay-Schultz, J., Cittelly, D. M., Hendricks, P., Patel, P., Kabos, P., Jacobsen, B. M., . . . Sartorius, C. A. (2015). Progesterone downregulation of miR-141 contributes to expansion of stem-like breast cancer cells through maintenance of progesterone receptor and Stat5a. Oncogene, 34(28), 3676.

Hong, L., Yang, J., Han, Y., Lu, Q., Cao, J., & Syed, L. (2012). High expression of miR-210 predicts poor survival in patients with breast cancer: a meta-analysis. Gene, 507(2), 135-138.

Hortobagyi, G. N., de la Garza Salazar, J., Pritchard, K., Amadori, D., Haidinger, R., Hudis, C. A., . . . Namer, M. (2005). The global breast cancer burden: variations in epidemiology and survival. Clinical breast cancer, 6(5), 391-401.

Huang, X., Le, Q.-T., & Giaccia, A. J. (2010). MiR-210–micromanager of the hypoxia pathway. Trends in molecular medicine, 16(5), 230-237.

Kota, S. K., & Balasubramanian, S. (2010). Cancer therapy via modulation of micro RNA levels: a promising future. Drug discovery today, 15(17-18), 733-740.

Lagos-Quintana, M., Rauhut, R., Lendeckel, W., & Tuschl, T. (2001). Identification of novel genes coding for small expressed RNAs. Science, 294(5543), 853-858.

Lee, R. C., & Ambros, V. (2001). An extensive class of small RNAs in Caenorhabditis elegans. Science, 294(5543), 862-864.

Liep, J., Kilic, E., Meyer, H. A., Busch, J., Jung, K., & Rabien, A. (2016). Cooperative effect of miR-141-3p and miR-145-5p in the regulation of targets in clear cell renal cell carcinoma. PloS one, 11(6), e0157801.

Liu, C., Liu, R., Zhang, D., Deng, Q., Liu, B., Chao, H. P., ... & Zhong, Y. (2017). MicroRNA-141 suppresses prostate cancer stem cells and metastasis by targeting a cohort of pro-metastasis genes. Nature communications, 8, 14270.

Mei, Z., He, Y., Feng, J., Shi, J., Du, Y., Qian, L., . . . Jie, Z. (2014). MicroRNA‐141 promotes the proliferation of non‐small cell lung cancer cells by regulating expression of PHLPP1 and PHLPP2. FEBS letters, 588(17), 3055-3061.

Meng, F., Henson, R., Wehbe–Janek, H., Ghoshal, K., Jacob, S. T., & Patel, T. (2007). MicroRNA-21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer. Gastroenterology, 133(2), 647-658.

Nafissi, N., Saghafinia, M., Motamedi, M. H. K., & Akbari, M. E. (2012). A survey of breast cancer knowledge and attitude in Iranian women. Journal of cancer research and therapeutics, 8(1), 46.

Negari, A. T., Emamvirdizadeh, A., Majdnia, M., Ahmadi, A. M., Shahbazi, F., Molaei, A., & hesamoddin Bidoki, S. (2018). KMT2D and IGF2 Genes Expression in Breast Cancer Patients. Age, 7, 20.

Puissegur, M. P., Mazure, N. M., Bertero, T., Pradelli, L., Grosso, S., Robbe-Sermesant, K., ... & Fourre, S. (2011). miR-210 is overexpressed in late stages of lung cancer and mediates mitochondrial alterations associated with modulation of HIF-1 activity. Cell death and differentiation, 18(3), 465.

Reis-Filho, J. S., & Pusztai, L. (2011). Gene expression profiling in breast cancer: classification, prognostication, and prediction. The Lancet, 378(9805), 1812-1823.

Rothe, F., Ignatiadis, M., Chaboteaux, C., Haibe-Kains, B., Kheddoumi, N., Majjaj, S., . . . Harris, A. L. (2011). Global microRNA expression profiling identifies MiR-210 associated with tumor proliferation, invasion and poor clinical outcome in breast cancer. PloS one, 6(6), e20980.

Rouzier, R., Perou, C. M., Symmans, W. F., Ibrahim, N., Cristofanilli, M., Anderson, K., . . . Wagner, P. (2005). Breast cancer molecular subtypes respond differently to preoperative chemotherapy. Clinical Cancer Research, 11(16), 5678-5685.

Siegel, R., Ma, J., Zou, Z., & Jemal, A. (2014). Cancer statistics, 2014. CA: a cancer journal for clinicians, 64(1), 9-29.

Tao, Z., Shi, A., Lu, C., Song, T., Zhang, Z., & Zhao, J. (2015). Breast cancer: epidemiology and etiology. Cell biochemistry and biophysics, 72(2), 333-338.

Uhlmann, S., Zhang, J., Schwäger, A., Mannsperger, H., Riazalhosseini, Y., Burmester, S., . . . Sahin, Ö. (2010). miR-200bc/429 cluster targets PLCγ1 and differentially regulates proliferation and EGF-driven invasion than miR-200a/141 in breast cancer. Oncogene, 29(30), 4297.



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