Investigation of ATG5, ATG12 and LC3 Genes Expression Related to Autophagy in Colorectal Cancer

Nasredin Moradi, Arezou Sabbagh hadi, Parisa Pilvar



Background: Colorectal cancer is the fourth leading cause of cancer deaths around the world. This type of cancer, like other cancers, is caused by the influence of environmental and genetic factors. One of the important mechanisms involved in cancer, especially colorectal cancer, is the autophagy mechanism. In this mechanism, important genes have been identified, including ATG12, ATG5, and LC3 genes.

Method: To evaluate the gene expression level, thirty colorectal tumor samples and thirty adjacent normal samples were used for carrying out Real-Time PCR method.

Results: We reported that there was no significant difference in the expression level of ATG12 and LC3 genes in tumor samples compared to normal samples (P>0.05), while the expression level of ATG5 gene with P Value=0.031 illustrated that this gene was reduced in colorectal tumor samples.

Key Words: Colorectal cancer, Autophagy, Real Time-PCR, ATG12, ATG5, LC3


Colorectal cancer, Autophagy, Real Time-PCR, ATG12, ATG5, LC3.

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