Investigation of ATG5, ATG12 and LC3 Genes Expression Related to Autophagy in Breast Cancer

Nasredin Moradi, Shohreh Zare Karizi, Mohammad Ebrahim Zadeh


Purpose: Breast cancer is caused by uncontrolled growth of the cell in the breast tissue, and the cancer is the most common cancer in women. Among the mechanisms involved in the incidence of cancer, the autophagy mechanism is recognized as one of these important pathways. Therefore, the study of effective genes in this pathway can be of great importance. The genes of LC3, ATG5 and ATG12 are among the important genes in this pathway.

Method: The Real Time-PCR method was used to evaluate the expression levels of LC3, ATG5 and ATG12 genes in 30 breast tissues and 30 adjacent normal tissues. Moreover, GenAll and Takara kits were used to extract RNA as well as cDNA synthesis, respectively.

Results:  The results of this study showed that there is no significant difference in ATG5, ATG12 and LC3 genes expression in tumor samples compared to normal samples (P> 0.05). The evaluation of expression changes in ATG5, ATG12 and LC3 demonstrate that, out of 30 tumor samples which were considered for each of genes, 18 tumor samples of ATG5 gene, 17 tumor samples of ATG12 gene and 16 tumor samples of LC3 gene illustrate the expression decreasing.

Conclusion: Finally, no significant changes were observed for the selected genes in this study. Since this research was designed on a pilot study, more researches and samples are needed to obtain more accurate results.


Breast cancer, Autophagy, Real Time-PCR, LC3, ATG5, ATG12.

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